Clinical and laboratory characteristics of herpes simplex virus infection in newborn infants

Cilj rada: analiza kliničkih i laboratorijskih karakteristika novorođenčadi sa infekcijom izazvanom Herpes simpleks virusom (HSV). Procena učestalosti neonatalnog herpesa (NH) u našoj sredini. Ispitivanje korelacije seroloških i molekularnih metoda dijagnostike. Definisanje kriterijuma za dijagnostičke i terapijske postupke. Materijal i metode: Analizirani su podaci o novorođenčadi i odojčadi uzrasta ≤45 dana sa sumnjom na infekciju HSV u periodu od januara 2003. do maja 2015. godine. Dokazana HSV infekcija je podrazumevala ispoljenu kliničku sliku NH i pozitivan PCR i/ili serologiju na HSV. Za ispitivanje incidencije NH korišćeni su podaci Republičkog zavoda za statistiku Srbije. Analizirane su kliničke manifestacije, laboratorijske analize, ishod lečenja, kao i kvatnifikovanje težine bolesti korišćenjem skorova SNAP II i SNAPPE II. Za statističku analizu korišćene su deskriptivne i analitičke metode. Rezultati: Studija ima 168 ispitanika, 29 sa dokazanom, 133 sa suspektnom i 6 sa asimptomskom HSV infekcijom. Incidencija NH tokom ispitivanog perioda je 3,01 na 100.000 živorođenih. U periodu 2010-2015. godine, sa uvođenjem rutinske PCR dijagnostike zabeležen je porast incidencije na 4,78 na 100 000 živorođenih. Najveći broj novorođenčadi sa dokazanom infekcijom, 72,45%, imao je herpesni encephalitis, 24,1% je imalo diseminovani oblik i 3,5% lokalizovani oblik bolesti. Većina infekcija uzrokovana je HSV-2 tipom (55,2%). Teži oblik bolesti imala su novorođenčad sa HSV-1 infekcijom. Ukupna smrtnost iznosila je 13,8%. Zaključak: Učestalost neonatalnog herpes u našoj populaciji odgovara učestalosti u ostalim evropskim zemljama. Sa unapređenjem dijagnostičkih metoda očekujemo dalji porast incidencije. Osobenosti NH u našoj sredini su veći broj HSV-2 infekcija i češća pojava herpes encefalitisa. Smrtni ishod je češći kod HSV-1 infekcije.Aim of study: Analysis of clinical and laboratory characteristics of newborns with Herpes simplex virus infection (HSV). Estimation of neonatal herpes incidence (NH) in our population. Correlation of serologycal and PCR tests. Comparison between suspected and confirmed cases of NH in order to define diagnostic and therapeutic protocol. Material and methods: Medical records of infants age ≤ 45 days with suspected NH, from January 2003 to May 2015. A confirmed case of NH was defined in an infant with clinical signs compatible with HSV disease and positive serology or PCR for HSV. Incidence was calculated by using Statistical Office of Republic of Serbia’s data of live births. We analyzed clinical manifestations, laboratory analysis, treatment outcome and predicting scores (SNAP II and SNAPPE II). Descriptive and analytic statistical methods were used for analysis of study results. Results: There were 168 infants, 133 with suspected, 29 with confirmed and 6 with asymptomatic HSV infection. Incidence of NH during the study period was 3.01 per 100.000 live births. Rise of incidence to 4.78 per live births 100.000 was noted after 2010. Majority of infants, 72.45%, presented with encephalitis, 24.1% had disseminated and 3,5% localized disease. Majority of infections were caused by HSV-2 (55.2%). Mortality was 13.8%, mostly accompanied with HSV-1 disease. Conclusion: Incidence of NH in our population is similar to other European countries. The rise of incidence was observed with improvement of HSV diagnostics. Characteristics of NH in our population are predominance of HSV-2 infections and herpes encephalitis. Mortality in our group is in accordance with other reports of NH

Finite element analysis of spherical storage tank stress state

In this paper, a spherical storage tank for the storage of liquefied petroleum gas is analysed. A design calculation of structural elements is performed according to valid standards and technical regulations. Seismic loads are determined. By using the finite element method, a structural analysis of the spherical storage tank is performed for the design case in which the storage tank is exposed to effects of self-weight of sphere with supporting pillars; complete reinforcement; staircases and platforms; snow; hydrostatic pressure of the working fluid; design pressure; and horizontal seismic forces. The zones with maximum stress values are shown and the obtained results are analysed

Finite element analysis of spherical storage tank stress state

In this paper, a spherical storage tank for the storage of liquefied petroleum gas is analysed. A design calculation of structural elements is performed according to valid standards and technical regulations. Seismic loads are determined. By using the finite element method, a structural analysis of the spherical storage tank is performed for the design case in which the storage tank is exposed to effects of self-weight of sphere with supporting pillars; complete reinforcement; staircases and platforms; snow; hydrostatic pressure of the working fluid; design pressure; and horizontal seismic forces. The zones with maximum stress values are shown and the obtained results are analysed

Statistical analysis of water quality parameters in the basin of the Nisava River (Serbia) in the period 2009-2018

This paper analyzes water quality from four hydrological stations in the Nisava river basin from 2009-2018 based on the following parameters: pH, conductivity, O-2 saturation, BOD5, suspended solids, total oxidized nitrogen, phosphates, turbidity and coliform bacteria. Authors have applied WQI (water quality index) as the most reliable indicator of the watercourses pollution for setting of surface water flow quality. Statistical analysis of variance (ANOVA) using t-test inferential statistical analysis and post-hoc Tukey test were applied to evaluate statistically significant differences between specific data groups. The results show that bad water quality was registered on all the profiles on an annual basis (WQI = 65-71). The river water pH decreases downstream, while values of BOD5, suspended solids, turbidity, TON, phosphates and coliform bacteria gradually increase. The waters of the Nisava at the station Nis belong to the third class of waters and they are mostly loaded with organic matter originating from waste sewage and industrial waters of both urban and rural areas

Istorijski razvoj Darsi-Vaisbahove jednačine za otpor strujanju fluida kroz cevovode

U članku je prikazan istorijski razvoj Darsi-Vaisbahove jednačine za otpor strujanju fluida kroz cevovode. Dat je kratki pregled evolucije izračunavanja koeficijenta trenja kroz doprinose niza istraživača i inženjera

Statistical analysis of temperature regime change on the example of Sokobanja basin in eastern Serbia

The changes and oscillations in air temperature during the second half of the 20th and in the early 21st century, have become one of the major concerns of almost all scientific disciplines. Such changes are noticeable both at the local and global level. The objective of this paper is to point out that the changes of this climate element can also be detected at the local level. The research underlying this paper is, in terms of territory, limited to the Sokobanja basin in Eastern Serbia. The analysis relies on the data on air temperature for the period 1946-2012 taken from the Meteorological weather station located in Sokobanja. The obtained data were processed in line with the recommendations of the World Meteorological Organization. The evidenced statistically significant changes in air temperature were examined using the following statistical tests: Pettit test, Standard Normal Homogeneity test, Buishand range test, and von Neumann test

The impact of Mediterranean oscillations on periodicity and trend of temperature in the valley of the Nisava river - a fourier and wavelet approach

Periodicity of temperature on three stations in the Nisava River valley in period 1949-2014, has been analyzed by means of Fourier and wavelet transforms. Combined periodogram based on fast Fourier transform shows considerable similarity among individual series and identffies significant periods on 2.2, 2.7, 3.3, 5, 6-7, and 8.2 years in all datasets. Wavelet coherence analysis connects strongest 6-7 years spectral component to Mediterranean oscillation, starting in 1980s. Combined periodogram of Mediterranean oscillation index reveals 6-7 years spectral component as a dominant mode in period 1949-2014. Wavelet power spectra and partial combined periodograms show absence of 6-7 years component before 1975, after which this component becomes dominant in the spectrum. Consistency between alternation in temperature trend in the Nisava River valley and change in periodicity of Mediterranean oscillation was found